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KMID : 0379520070230030223
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한국독성학회지
2007 Volume.23 No. 3 p.223 ~ p.229
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Effects of Diglyceride-Conjugated Linoleic Acid on Proliferation and Differentiation of 3T3-L1 Cells
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Jeong Jae-Hwang
Lee Sang-Hwa
Hue Jin-Joo
Lee Yea-Eun
Lee Young-Ho
Hong Soon-Ki
Jeong Seong-Woon
Nam Sang-Yoon
Yun Young-Won
Lee Beom-Jun
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Abstract
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Conjugated linoleic acid (CLA) has been recently reported to have an anti-obesity effect in animals and humans. The objective of this study was to investigate effects of diglyceride (DG)-CLA on proliferation and differentiation of 3T3-L1 preadipocytes. Cell proliferation was determined using WST-8 analysis and cell differentiation was determined by glycerol-3-phosphate dehydrogenase (GPDH) activity. Lipid accumulation in differentiating 3T3-L1 cells was determined by Oil red O staining. There were four experimental groups including vehicle control (DMSO), CLA, triglyceride (TG)-CLA, and DG-CLA. Treatments of CLA, TG-CLA, and DG-CLA at the concentrations of 10~1000 ㎍/㎖ reduced proliferation of preconfluent 3T3-L1 cells in a dose-dependent manner. Among them CLA was the most effective in the proliferation inhibition of preconfluent 3T3-L1 cells with increasing concentrations. Treatments of CLA and DG-CLA at the concentration of 100 ㎍/㎖ significantly inhibited differentiation of postconfluent 3T3-L1 cells as measured by GPDH activity (p＜0.05). In addition, treatments of CLA, TG-CLA, and DG-CLA effectively inhibited lipid accumulation during differentiation of 3T3-L1 cells. DG-CLA had the most inhibitory effect on the differentiation and lipid accumulation. These results suggest that the compounds including CLA have a respectable anti-obesity effect and that consumption of DG-CLA as a dietary oil may give a benefit for controlling overweight in humans.
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KEYWORD
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Conjugated linoleic acid, Diglyceride, Glycerol-3-phosphate dehydrogenase, Obesity, Triglyceride, 3T3-L1 cells
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